Development of Rapid-Buffer Exchange ATR-FTIR Spectroscopy for Membrane Proteins
نویسندگان
چکیده
منابع مشابه
Development of a rapid Buffer-exchange system for time-resolved ATR-FTIR spectroscopy with the step-scan mode
Attenuated total reflectance (ATR)-FTIR spectroscopy has been widely used to probe protein structural changes under various stimuli, such as light absorption, voltage change, and ligand binding, in aqueous conditions. Time-resolved measurements require a trigger, which can be controlled electronically; therefore, light and voltage changes are suitable. Here we developed a novel, rapid buffer-ex...
متن کاملRapid discrimination of maggots utilising ATR-FTIR spectroscopy.
Entomological evidence is used in forensic investigations to indicate time since death. The species and age of maggots or flies that are present at the scene can be used when estimating how much time has passed since death. Current methods that are used to identify species and developmental stage of larvae and fly samples are highly subjective, costly and often time consuming processes and requ...
متن کاملInteraction of Two Different Types of Membrane Proteins with Model Membranes Investigated by FTIR ATR Spectroscopy
Polarized FTIR ATR spectroscopy was used to investigate the interaction of mitochondrial creatine kinase (Mi-CK) and intestinal alkaline phosphatase (AP) with model membrane assemblies. Mi-CK was immobilized by adsorption to the negatively charged cardiolipin (CL) leaflet of a supported CL/DPPA bilayer. H-D-exchange of the enzyme and the stability under flowthrough conditions of the protein/mem...
متن کاملEvaluating the Role of antioxidants in the stabilization of hydroxypropyl cellulose by ATR-FTIR Spectroscopy
This study is aimed at evaluating the performance of tetrakismethylene-3-3,5-di-tert-butyl-4-hydroxyphenyl propionate methane S1010, tris2,4-di-tert-butylphenyl phosphite S1680 and ascorbic acid AA antioxidants in stabilization of hydroxypropyl cellulose HPC. For this purpose, a number of films with weight percent ratios of 0.4 w/w were prepared in a 1 isopropanol solution w/v. Then, the films ...
متن کاملThe early development and application of FTIR difference spectroscopy to membrane proteins: A personal perspective
Membrane proteins facilitate some of the most important cellular processes including energy conversion, ion transport and signal transduction. While conventional infrared absorption provides information about membrane protein secondary structure, a major challenge is to develop a dynamic picture of the functioning of membrane proteins at the molecular level. The introduction of FTIR difference ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Seibutsu Butsuri
سال: 2014
ISSN: 0582-4052,1347-4219
DOI: 10.2142/biophys.54.272